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KMID : 1134820190480121330
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 12 p.1330 ~ p.1336
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Protective Roles of Polysaccharides Extracted from Cudrania tricuspidata Fruits in Macrophages
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Byun Eui-Baek
Kim Kwang-Wook
Oh Nam-Soon
Park Won-Jeong
Kim Woo-Sik
Song Ha-Yeon
Han Jeong-Moo
Byun Eui-Hong
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Abstract
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It is believed that Cudrania tricuspidata fruits polysaccharides extracts (CTPS) have antioxidant, anti-inflammatory, anti-cancer, and immune activity effects. However, there have been no studies that have shown the cytoprotective effects of CTPS. Therefore, the present study was conducted to confirm the cytoprotective effect of CTPS in terms of their inhibition of cytotoxicity, caspase activity, reactive oxygen species (ROS) production, and mitochondrial outer membrane permeability (MOMP) increase in Raw264.7. Macrophage apoptosis was induced by staurosporine (STS) treatment. CTPS treatment significantly reduced STS induced cell death and annexin V positive cell levels in a dose-dependent manner. In addition, CTPS treatment inhibited activation of caspase-3, 8, 9, and PARP in macrophages. Further, CTPS treatment reduced STS induced ROS production and MOMP increase in macrophages. In conclusion, CTPS treatment inhibits STS-induced macrophage apoptosis through inhibition of cytotoxicity, caspase activation, ROS production, and MOMP increase. These results suggest CTPS could be a potential candidate for treatment of immune cell protection effects.
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KEYWORD
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Cudrania tricuspidata, macrophage, apoptosis, protective roles
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